Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Morphological changes of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p) after 24 h treatment.

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Apoptosis of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Cell cycle analysis of A549, A431, HL60, and IMR90 cell lines untreated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Cell Cycle Assay, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Caspase-3 expression of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m), treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Percentage Cell Viability of A549, A431, HL60 and, IMR90 Cell-Lines Treated with Various Concentrations of SNP-B and SNP-C a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Control

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Apoptotic Study (i.e., Annexin V/PI Expression Study) of SNP-B and SNP-C against A549, A431, HL60, and IMR90 Cell-Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Cell Cycle Analysis of SNP-B and SNP-C against A549, A431 HL60, and IMR90 Cell Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Cell Cycle Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Caspase-3 Expression Study of SNP-B and SNP-C against A549, A431, HL60, and IMR90 Cell Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Fluorescence

Journal: Biomedicines

Article Title: The Thioredoxin System of Mammalian Cells and Its Modulators

doi: 10.3390/biomedicines10071757

Figure Lengend Snippet: Cytotoxicity of TrxR inhibitors.

Article Snippet: 3. Organoselenium compounds , 3.1. Organo- selenium compounds 3.2. Phenylarsenic oxide derivatives (dithiarsanes) , 1,2-(5,5′-Dimethoxy(bis-1,2-benzisoselenazol-3(2 H )-one))ethane 2-(4-Aminophenyl)-1,3,2-dithiarsenane (PAO−PDT) , , 1.64 0.6 , U-87MG ATCC HL-60 , [ ] [ ] .

Techniques: Analogues

Journal: Immunology

Article Title: Functional exhaustion of CD4 + T cells induced by co‐stimulatory signals from myeloid leukaemia cells

doi: 10.1111/imm.12665

Figure Lengend Snippet: Up‐regulation of surface molecules related to activation and/or exhaustion on CD4 + T cells co‐cultured with different myeloid leukaemia cell lines for 96 hr under continuous stimuli conditions

Article Snippet: Human myeloid leukaemia cell lines, KG‐1, Kasumi‐1, HL‐60, U937 and THP‐1 were either obtained from the American Type Culture Collection (ATCC, LGC Promochem, Rockville, MD) or received as kind gifts.

Techniques: Activation Assay, Expressing

Journal: The Journal of Biological Chemistry

Article Title: Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation *

doi: 10.1074/jbc.M115.702787

Figure Lengend Snippet: Step-by-step protocol applied to screen the library of bioactive compounds at The Broad Institute

Article Snippet: Human promyelocytic leukemia HL60 cells (Sigma) differentiated into neutrophil-like cells by all- trans -retinoic acid were used as the HTS-compatible source of Nox2 model system for screening Nox2 inhibitors ( 25 , 26 ).

Techniques: Cell Culture, Concentration Assay, Inhibition, Fluorescence

Journal: The Journal of Biological Chemistry

Article Title: Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation *

doi: 10.1074/jbc.M115.702787

Figure Lengend Snippet: Positive hits obtained during screening of the library of bioactive compounds using three probes as follows: hydropropidine (50 μ m ) in the presence of DNA (0.1 mg/ml) as a probe for Ȱ, and coumarin boronic acid (100 μ m ) or Amplex Red (50 μ m ) in the presence of HRP (0.1 units/ml) as probes for H 2 O 2 Differentiated HL60 cells were stimulated with PMA (1 μ m ) to induce Nox2 activity in HBSS supplemented with HEPES buffer (25 m m ) and dtpa (0.1 m m ).

Article Snippet: Human promyelocytic leukemia HL60 cells (Sigma) differentiated into neutrophil-like cells by all- trans -retinoic acid were used as the HTS-compatible source of Nox2 model system for screening Nox2 inhibitors ( 25 , 26 ).

Techniques: Activity Assay

Journal: The Journal of Biological Chemistry

Article Title: Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation *

doi: 10.1074/jbc.M115.702787

Figure Lengend Snippet: IC 50 values for selected positive hits, determined by monitoring the effect of the compounds on the rate of oxidation of coumarin boronic acid (100 μ m ) as a probe for H 2 O 2 Differentiated HL60 cells were stimulated with PMA (1 μ m ) to induce Nox2 activity in HBSS supplemented with HEPES buffer (25 m m ) and dtpa (0.1 m m ) and the extent of probe oxidation was monitored in 384-well plates using fluorescence plate reader.

Article Snippet: Human promyelocytic leukemia HL60 cells (Sigma) differentiated into neutrophil-like cells by all- trans -retinoic acid were used as the HTS-compatible source of Nox2 model system for screening Nox2 inhibitors ( 25 , 26 ).

Techniques: Activity Assay, Fluorescence